RNA
GreenFET1

Part:BBa_K1586000:Design

Designed by: Exeter iGEM 2015   Group: iGEM15_Exeter   (2015-09-10)

Synthetic toehold riboswitch - J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 769


Design Notes

The first 12 nucleotides of the toehold (not including the GGG leader sequence) should not form a part of the toehold stem.

The majority of the RBS should be within the loop region of the toehold.

There must not be an inframe stop codon within the main linker sequence.

The linker sequence length must be a multiple of three.

For a more in depth explanation, see the design page of our wiki: http://2015.igem.org/Team:Exeter/Toehold_Design


Source

The sequence of this part was designed by and characterised in cells by Green et al. 2014 (http://www.sciencedirect.com/science/article/pii/S0092867414012896; supplementary information, table 3, Forward Engineered Toehold 1).

References